Rice Transcriptome Analysis Reveals Nitrogen Starvation Modulates Differential Alternative Splicing and Transcript Usage in Various Metabolism-Related Genes

Nitrogen (N) is crucial for plant growth and development; however, excessive use of N fertilizers cause many problems including environmental damage, degradation soil fertility, high cost to the farmers. Therefore, immediate implementation required develop efficient crop varieties. Rice being low nitrogen efficiency (NUE) a demand staple food across world has become favorite study NUE trait. In current study, we used publicly available transcriptome data generated from root shoot tissues two rice genotypes IR-64 Nagina-22 (N-22) under optimum supply (N+) chronic N-starvation (N−). A stringent pipeline was applied detect differentially expressed genes (DEGs), alternatively spliced (DAS) genes, transcripts (DETs) differential transcript usage (DTU) in both varieties N+ N− conditions. The DAS DTU identified were found be involved several metabolic biosynthesis processes. We suggest alternative splicing (AS) plays an important role fine-tuning regulation pathways related genotype, tissue, condition-dependent manner. will help understanding transcriptional dynamics traits future.